TUTORIAL

Calculating your dosage

A simple, precise method to determine solution concentration and the volume to draw for your laboratory protocol.

1. 1

   Know the concentration

   Concentration is the amount of peptide in each milliliter of solution after reconstitution.

   Peptide

   ÷

   Water (ml)

   =

   mg / mlConcentration

   Example

   40 mg peptide + 3 ml bacteriostatic water → 13.33 mg/ml

   40 ÷ 3 = 13.33

2. 2

   Set the protocol dose (mg)

   Refer to your research protocol for the amount of peptide wanted per draw, expressed in milligrams.

   e.g. 1 mg per draw
3. 3

   Choose the measuring tool

   In the lab, volume is most often measured with a graduated syringe. Some protocols also use a dosing pen calibrated in “clicks”.

   Syringe

   Direct reading in ml (or units on a U-100 syringe: 100 units = 1 ml).

   Dosing pen

   e.g. 3 ml pen = 300 clicks → 1 click = 0.01 ml.

4. 4

   Calculate the volume

   Once concentration is known, convert the protocol dose into the volume to draw.

   Base formula (syringe)

   dose (mg)÷concentration (mg/ml)=volume (ml)

   Dosing pen option (3 ml / 300 clicks)

   volume (ml)×300=clicks
5. 5

   Full example

   40 mg peptide reconstituted with 3 ml → 13.33 mg/ml. Protocol dose: 1 mg.

   1 mg÷ 13.330.075 ml

   U-100 syringe

   0.075 ml ≈ 7.5 units

   3 ml pen

   0.075 × 300 ≈ 22.5 clicks

Technical guide for laboratory use only. Not medical advice. Examples are fictional and provided for teaching purposes.