TUTORIAL

Reconstituting your peptides

SanaPept peptides arrive lyophilized (powder). Here is a typical laboratory workflow to bring them into solution with bacteriostatic water — clean, precise, and safe.

1. 1

   Gather the materials

   • • Lyophilized peptide vial
   • • Bacteriostatic water
   • • Suitable sterile syringe + needle
   • • Alcohol wipes, gloves, clean surface

   Peptide

   Bac Water

   Syringe

   Wipes
2. 2

   Prepare the vials

   • • Remove the protective caps from both vials
   • • Check that stoppers are intact and clean
   • • Calculate the water volume for your protocol (see the dosage guide)

   Cap

   →

   Stopper ready

   Tip

   Write the target concentration on a label now (e.g. 5 mg + 2 ml → 2.5 mg/ml).

3. 3

   Disinfect

   • • Put on clean gloves
   • • Wipe both vial stoppers with an alcohol wipe
   • • Let dry a few seconds before piercing

   Gloves + wipe
4. 4

   Draw bacteriostatic water

   • • First draw an air volume equal to the desired water volume
   • • Inject the air into the water vial, then draw the water
   • • Expel air bubbles from the syringe

   Bac Water

   +

   Exact volume

   Important

   Use only bacteriostatic water suited for laboratory reconstitution — not tap water, and not unpreserved sterile water if your protocol calls for Bac Water.

5. 5

   Inject water into the peptide

   • • Pierce the peptide vial stopper
   • • Let the water run slowly down the wall, not as a direct jet onto the powder
   • • Let it rest for a moment

   Slow injection

   →

   Along the wall

   Tip

   Injecting slowly reduces foaming and protects the peptide.

6. 6

   Dissolve the peptide

   • • Gently roll the vial between your fingers (circular motion)
   • • Wait for a clear, homogeneous solution
   • • Do not use a violent vortex

   Gentle swirl

   Do not shake

   Shaking can denature the peptide. Patience and a gentle swirl are usually enough.

7. 7

   Store correctly

   • • Refrigerate according to your protocol (often 2–8 °C once reconstituted)
   • • Protect from light if recommended
   • • Label: peptide, concentration, reconstitution date

   Cold storage

   Keep cold

   Lyophilized powder keeps better dry and cold; once in solution, follow your protocol timelines strictly.

8. 8

   Dispose safely

   • • Place needles and syringes in a suitable sharps container
   • • Never throw needles in household trash
   • • Follow your lab / facility rules

   Sharps bin

   Safety

Technical guide for laboratory use only. Not medical advice. Follow sterility best practices for your research environment.